Resources by Category: "q-PCR"

Inhibition of peroxisome proliferator-activated receptor Γ: a potential link between chronic maternal hypoxia and impaired fetal growth

Julian, Colleen G, Ivana V Yang, Vaughn a Browne, Enrique Vargas, Carmelo Rodriguez, Brent S Pedersen, Lorna G Moore, and David a Schwartz. 2013. “Inhibition of Peroxisome Proliferator-Activated Receptor Γ: a Potential Link Between Chronic Maternal Hypoxia and Impaired Fetal Growth.” FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology 28 (Published online… Read more »

NovaPlex™ for Amplification and Analysis of Nucleic Acids from Formalin-Fixed, Paraffin-Embedded Tissue

T. Tesmer, M. Mastronardi, E. Kamberov, J. Langmore The fixation of human tissues with formalin, and their subsequent embedding in paraffin, has been a routine method of collecting and preserving surgical specimens for many years. These formalin-fixed, paraffin-embedded (FFPE) tissues represent an extremely valuable resource for investigators interested in determining both the pattern of gene… Read more »

Two Methods for High-Throughput NGS Template Preparation for Small Clinical Samples Without Automation

J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor) Clinical samples are difficult to prepare for NGS, because of the small amounts of formalin-fixed tissue, plasma, urine, and single-cell DNA available from patients. Conventional whole genome amplification methods are too biased for NGS applications, and the existing NGS preparation kits require intermediate… Read more »

Quantitative Quality Metrics for NGS Libraries from Clinical Samples

J. Langmore, E. Kamberov, T. Tesmer, J. Jessmon, M. Carey, I. King (Rubicon Genomics, Ann Arbor) Clinical samples are difficult to prepare for NGS, because they are often present in small amounts, are highly degraded, and are of small size. We have tried to characterize clinical samples, suggest the effects of those characteristics on NGS… Read more »