Resources by Category: "Sequencing"

Large conserved domains of low DNA methylation maintained by Dnmt3a.

Mira Jeong, Deqiang Sun, Min Luo, Yun Huang, Grant A Challen, Benjamin Rodriguez, Xiaotian Zhang, Lukas Chavez, Hui Wang, Rebecca Hannah, Sang-Bae Kim, Liubin Yang, Myunggon Ko, Rui Chen, Berthold Göttgens, Ju-Seog Lee, Preethi Gunaratne, Lucy A Godley, Gretchen J Darlington, Anjana Rao, Wei Li & Margaret A Goodell. Nat Genet. 2014 Jan;46(1):17-23. doi: 10.1038/ng.2836. Epub 2013 Nov 24.

ThruPLEX™ Technology Repairs Damage and Amplifies Small Amounts of Biofluid DNA for NGS, Microarrays and PCR

M. Mastronardi, T. Tesmer, E. Kamberov, J. Langmore Biofluid Challenges: Biofluids, including plasma, serum, urine and CSF, contain valuable biomarkers that are used in research and diagnostics. These matrices present many challenges to traditional sample preparation methods. Biofluid DNA is fragmented (100-200 bp) and very dilute (e.g., 1-20 ng/mL of plasma). Additionally, the biomarker-containing DNA… Read more »

NovaPlex™ for Amplification and Analysis of Nucleic Acids from Formalin-Fixed, Paraffin-Embedded Tissue

T. Tesmer, M. Mastronardi, E. Kamberov, J. Langmore The fixation of human tissues with formalin, and their subsequent embedding in paraffin, has been a routine method of collecting and preserving surgical specimens for many years. These formalin-fixed, paraffin-embedded (FFPE) tissues represent an extremely valuable resource for investigators interested in determining both the pattern of gene… Read more »

Rapid Genome Sequencing of RNA Viruses

Mizutani Tetsuya, Endoh Daiji, Okamoto Michiko, Shirato Kazuya, Shmizu Hiroyuki, Arita Minertaro, Fukushi Shuetsu, Saijo Masayuki, Sakai Kouji, Lim Chang Kweng, Ito Mikako, Nerome Reiko, Takasaki Tomohiko, Ishii Koji, Suzuki Tetsuro, Kurane Ichiro, Morikawa Shigeru, Nishimura Hidekazu. Emerging Infectious Diseases, www.cdc.gov/eid, 2007 Feb; 13(2):322-324