K. Shazand, J. Ning, E. Ranghini, A. Popkie, and J.P. Jerome The ability to detect rare alleles is key to the discovery and characterization of mutations in cancer research. These mutations are found at low frequencies, mainly because of tumor heterogeneity and tumor DNA dilution in the germline background. Cell-free DNA (cfDNA) in plasma provides… Read more »
Resources by Category: "Sequencing"
Deleye, L., De Coninck, D., Christodoulou, C., Sante, T., Dheedene, A., Heindryckx, B., et al. Whole genome amplification with SurePlex results in better copy number alteration detection using sequencing data compared to the MALBAC method.Sci Rep. 2015;5:11711. DOI: 10.1038/srep11711.
Fong, C. Y., Gilan, O., Lam, E. Y., Rubin, A. F., Ftouni, S., Tyler, D., Stanley, et al. BET inhibitor resistance emerges from leukaemia stem cells.Nature. 2015;525(7570):538-42. DOI: 10.1038/nature14888.
Liu C., Yu Y., Liu F., Wei X., Wrobel J.A., Gunawardena H.P., Zhou L., Jin J., Chen X. A chromatin activity-based chemoproteomic approach reveals a transcriptional repressome for gene-specificsilencing. Nature Communications. 2014 Dec 15;5:5733. DOI: 10.1038/ncomms6733.
Sachsenröder, J., Twardziok, S. O., Scheuch, M., & Johne, R. (2014). The General Composition of the Faecal Virome of Pigs Depends on Age, but Not on Feeding with a Probiotic Bacterium. PLoS ONE, 9(2), e88888. DOI:10.1371/journal.pone.0088888
Kim, Tae-Hee, Fugen Li, Isabel Ferreiro-Neira, Li-Lun Ho, Annouck Luyten, Kodandaramireddy Nalapareddy, Henry Long, Michael Verzi, and Ramesh a Shivdasani. 2014. “Broadly Permissive Intestinal Chromatin Underlies Lateral Inhibition and Cell Plasticity.” Nature (January 12). DOI:10.1038/nature12903.
Housheng Hansen, He Clifford, A Meyer, Sheng’en Shawn Hu, Mei-Wei Chen, Chongzhi Zang, Yin Liu, Prakash K Rao, Teng Fe, Han Xu, Henry Long, X Shirley Liu & Myles Brown. Nat Methods. 2014 Jan;11(1):73-8. doi: 10.1038/nmeth.2762. Epub 2013 Dec 8.
Mira Jeong, Deqiang Sun, Min Luo, Yun Huang, Grant A Challen, Benjamin Rodriguez, Xiaotian Zhang, Lukas Chavez, Hui Wang, Rebecca Hannah, Sang-Bae Kim, Liubin Yang, Myunggon Ko, Rui Chen, Berthold Göttgens, Ju-Seog Lee, Preethi Gunaratne, Lucy A Godley, Gretchen J Darlington, Anjana Rao, Wei Li & Margaret A Goodell. Nat Genet. 2014 Jan;46(1):17-23. doi: 10.1038/ng.2836. Epub 2013 Nov 24.
M. Mastronardi, T. Tesmer, E. Kamberov, J. Langmore Biofluid Challenges: Biofluids, including plasma, serum, urine and CSF, contain valuable biomarkers that are used in research and diagnostics. These matrices present many challenges to traditional sample preparation methods. Biofluid DNA is fragmented (100-200 bp) and very dilute (e.g., 1-20 ng/mL of plasma). Additionally, the biomarker-containing DNA… Read more »
T. Tesmer, M. Mastronardi, E. Kamberov, J. Langmore The fixation of human tissues with formalin, and their subsequent embedding in paraffin, has been a routine method of collecting and preserving surgical specimens for many years. These formalin-fixed, paraffin-embedded (FFPE) tissues represent an extremely valuable resource for investigators interested in determining both the pattern of gene… Read more »