TransPLEX® C-WTA Kit

Transcriptome Analysis for Clinical Use

transplex

Rubicon’s TransPLEX RNA amplification technology for FFPE samples was a standout in our evaluation of the available options for use with our Symphony products for the diagnosis and management of breast cancer,”

—David Macdonald, CEO of Agendia.

Is your TransPLEX Whole Transcriptome Analysis assay ready to move to the clinic?

TransPLEX C-WTA Kits are optimized for amplification of RNA from difficult samples such as FFPE and serum. The reagent formulations have been designed and optimized to yield a large library from as little as 1 ng of input RNA.

Transplex® C-WTA Kit is available for diagnostic use and is manufactured under cGMP.

*Protected by US Patent 7,718,403 and EP1604040

  • Proven technology with simple workflow that can be automated
  • Generates isothermal library without 3’-bias followed by amplification
  • Amplifies 10 ng – 300 ng of RNA or formalin-fixed RNA for microarray, PCR. or sequencing assays
  • High success rate- >90%- with FFPE specimens
  • Manufactured under cGMP

 TransPLEX Workflow is Readily Adaptable to Clinical Applications

transplex_workflow_chart

TransPLEX Graph

TransPLEX Graph
TransPLEX Graph

A total of 52 archived FFPE samples were amplified with the TransPLEX C-WTA kit or NuGen Ovation® V2 kit and expression analysis performed by microarrays. Samples prepared with the TransPLEX C-WTA kit shows a strong concordance (62%) of clinically-relevant genes with fresh-frozen samples. In addition, the TransPLEX C-WTA kit demonstrated greater concordance than the NuGen Ovation v2 Kit with all genes as well as those up- and down- regulated

Store at -20 °C.
Guaranteed for 9 months at -20°C  in a constant temperature freezer.

 

For diagnostic applications using human, animal, or plant sourced material; contact Business Development.
TransPLEX Whole Transcriptome Analysis Kits are available for research use from Sigma-Aldrich.

 

Performance

TransPLEX Graph

TransPLEX Graph
TransPLEX Graph

A total of 52 archived FFPE samples were amplified with the TransPLEX C-WTA kit or NuGen Ovation® V2 kit and expression analysis performed by microarrays. Samples prepared with the TransPLEX C-WTA kit shows a strong concordance (62%) of clinically-relevant genes with fresh-frozen samples. In addition, the TransPLEX C-WTA kit demonstrated greater concordance than the NuGen Ovation v2 Kit with all genes as well as those up- and down- regulated

Specifications

Store at -20 °C.
Guaranteed for 9 months at -20°C  in a constant temperature freezer.

 

Resources

Ordering Information

For diagnostic applications using human, animal, or plant sourced material; contact Business Development.
TransPLEX Whole Transcriptome Analysis Kits are available for research use from Sigma-Aldrich.