TransPLEX C-WTA Kit Provides Clinically Relevant Data

A total of 52 archived FFPE samples were amplified with the TransPLEX C-WTA kit or NuGen Ovation® V2 kit and expression analysis performed by microarrays. Samples prepared with the TransPLEX C-WTA kit shows a strong concordance (62%) of clinically-relevant genes with fresh-frozen samples. In addition, the TransPLEX C-WTA kit demonstrated greater concordance than the NuGen Ovation v2 Kit with all genes as well as those up- and down- regulated

TransPLEX C-WTA Kit Workflow

TransPLEX C-WTA Kit Technology

TransPLEX whole transcriptome amplification technology uses isothermal quasi-random primed library amplification followed by PCR amplification to produce up to 5 µg of total RNA suitable for both microarray and qPCR analyses in 5 hours.

  • Proven technology with simple workflow that can be automated
  • Generates isothermal library without 3′-bias followed by amplification
  • Amplifies 10 ng – 300 ng of RNA or formalin-fixed RNA for microarray, PCR, or sequencing assays
  • High success rate (>90%) with FFPE Specimens
  • Manufactured under cGMP

Store at -20 °C.
Guaranteed for 9 months at -20°C  in a constant temperature freezer.

For diagnostic applications using human, animal, or plant sourced material; contact Business Development.
TransPLEX Whole Transcriptome Analysis Kits are available for research use from Sigma-Aldrich.

Performance

TransPLEX C-WTA Kit Provides Clinically Relevant Data

A total of 52 archived FFPE samples were amplified with the TransPLEX C-WTA kit or NuGen Ovation® V2 kit and expression analysis performed by microarrays. Samples prepared with the TransPLEX C-WTA kit shows a strong concordance (62%) of clinically-relevant genes with fresh-frozen samples. In addition, the TransPLEX C-WTA kit demonstrated greater concordance than the NuGen Ovation v2 Kit with all genes as well as those up- and down- regulated

TransPLEX C-WTA Kit Workflow

TransPLEX C-WTA Kit Technology

TransPLEX whole transcriptome amplification technology uses isothermal quasi-random primed library amplification followed by PCR amplification to produce up to 5 µg of total RNA suitable for both microarray and qPCR analyses in 5 hours.

Advantages

  • Proven technology with simple workflow that can be automated
  • Generates isothermal library without 3′-bias followed by amplification
  • Amplifies 10 ng – 300 ng of RNA or formalin-fixed RNA for microarray, PCR, or sequencing assays
  • High success rate (>90%) with FFPE Specimens
  • Manufactured under cGMP

Specifications

Store at -20 °C.
Guaranteed for 9 months at -20°C  in a constant temperature freezer.

For diagnostic applications using human, animal, or plant sourced material; contact Business Development.
TransPLEX Whole Transcriptome Analysis Kits are available for research use from Sigma-Aldrich.

Resources

Overview

TransPLEX® C-WTA Kit is optimized for the amplification of RNA from difficult samples such as FFPE and serum, providing uniform representation across the entire transcript. The reagent formulations have been designed and optimized to yield a large library from as little as 10 ng of input RNA from fresh tissues and cells or 25 ng from FFPE. The complete TransPLEX C-WTA workflow is performed in a single tube, reducing workflow and minimizing risk of contamination or sample misidentification.

C-WTA Kit is available for diagnostic use only; cGMP certification ensures ready use for clinical assays.

Protected by US Patent 7,718,403 and EP1604040

*For commercial use only