journals December 30, 2016: How can you use Rubicon Genomics’                          Technology in your research?

The technology behind Rubicon Genomics’ NGS library preparation kits is used to produce high quality Illumina® NGS libraries using a simple, single tube, three step workflow. Publications in top journals were released this year showing how our technology can be used in a variety of applications including ChIP-seq, RNA-seq, FFPE, single cell sequencing, CNV detection and more. Recently, a paper published in BMC Plant Biology used our ThruPLEX® DNA-seq library preparation kit to reveal the effects of cytokinin on gene expression in shoots and roots of O. sativa (rice) by using NGS-based whole transcriptome analysis. To analyze gene expression in response to the cytokinin benzyladenine (BA) in roots and shoots, ThruPLEX DNA-seq was used to generate sequencing-ready libraries from cDNA prepared from rice seedling treated with BA.(READ MORE)

 

 

27915588_lNovember 18, 2016: How Deep Should I sequence? 

With the launch of ThruPLEX® Tag-seq, there are questions about how deep you need to sequence to detect the Minor Allele Frequency (MAF) of interest. In order to determine the detectable MAF there are several factors to consider, including input amount, depth of sequencing and capture panel size. ThruPLEX Tag-seq provides confident MAF detection by including 16 million unique molecular tags (UMTs) to label each DNA molecule. Using the UMTs, bioinformatics software groups the duplicates into amplification families and constructs a consensus sequence, thus reducing false positives. (READ MORE)

 

 

 

UMT video image

October 6, 2016: What are Unique Molecular Tags? 

Recently, Rubicon Genomics announced exciting news at AGBT’s Precision Medicine Conference–the launch of our ThruPLEX® Tag-seq Kit, a NGS library preparation kit with unique molecular tags (UMTs)! You might be thinking to yourself, “That’s great, but what are UMTs and why would I need them?” I’m here to help! Today I’m going to provide more details to help you understand what UMTs are, how they work and how they can be applied to your research. (READ MORE)