Resources by Category: "CTC"

Multifocal clonal evolution characterized using circulating tumour DNA in case of metastatic breast cancer

Muhammed Murtaza, Sarah-Jane Dawson, Katherine Pogrebniak, Oscar M. Rueda, Elena Provenzano, John Grant, Suet-Feung Chin, Dana W. Y. Tsui, Francesco Marass, Davina Gale, H. Raza Ali, Pankti Shah, Tania Contente-Cuomo, Hossein Farahani, Karey Shumansky, Zoya Kingsbury, Sean Humphray, David Bentley, Sohrab P. Shah, Matthew Wallis et al. Nature Communications 6, Article number: 8760 DOI:10.1038/ncomms9760  

Experience with 2 methods to simplify NGS Preps of clinical samples, including FFPE tissue, plasma, immunoprecipitates and single cells

J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor) Formalin-fixed tissue, FNA, plasma, urine, CSF and other clinical samples were standardized for many scientific and practical reasons long before the value and limitations of genetic testing were known. These clinical samples are highly degraded, and the analytes present in limiting quantities (e.g.,… Read more »

Quantitative Quality Metrics for NGS Libraries from Clinical Samples

J. Langmore, E. Kamberov, T. Tesmer, J. Jessmon, M. Carey, I. King (Rubicon Genomics, Ann Arbor) Clinical samples are difficult to prepare for NGS, because they are often present in small amounts, are highly degraded, and are of small size. We have tried to characterize clinical samples, suggest the effects of those characteristics on NGS… Read more »

Challenges and Solutions for Preparing NGS libraries from Clinical Samples

J. Langmore, E. Kamberov, T. Tesmer, J. Jessman, M. Carey (Rubicon Genomics, Ann Arbor) Clinical samples are difficult to prepare for NGS. Plasma, serum, urine, single cancer and fetal cells, formalin-fixed tissue, and even fresh tissue are collected as small samples ofvariable quantity and quality. To obtain high-quality, reproducible results, the enzymology and chemistry of… Read more »