Resources by Category: "Plasma"

Inhibition of peroxisome proliferator-activated receptor Γ: a potential link between chronic maternal hypoxia and impaired fetal growth

Julian, Colleen G, Ivana V Yang, Vaughn a Browne, Enrique Vargas, Carmelo Rodriguez, Brent S Pedersen, Lorna G Moore, and David a Schwartz. 2013. “Inhibition of Peroxisome Proliferator-Activated Receptor Γ: a Potential Link Between Chronic Maternal Hypoxia and Impaired Fetal Growth.” FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology 28 (Published online… Read more »

ThruPLEX™ Technology Repairs Damage and Amplifies Small Amounts of Biofluid DNA for NGS, Microarrays and PCR

M. Mastronardi, T. Tesmer, E. Kamberov, J. Langmore Biofluid Challenges: Biofluids, including plasma, serum, urine and CSF, contain valuable biomarkers that are used in research and diagnostics. These matrices present many challenges to traditional sample preparation methods. Biofluid DNA is fragmented (100-200 bp) and very dilute (e.g., 1-20 ng/mL of plasma). Additionally, the biomarker-containing DNA… Read more »

Comparison of Two New Methods to Increase the Sensitivity of Next Generation Sequencing for ChIP-seq, Methyl-seq and other Sonicated DNA Applications as well as for Short DNA in Non-Invasive Prenatal and Cancer Diagnostics

American Society of Human Genetics  2011 (Poster – PDF) E. Kamberov, J. Langmore, T. Tesmer1, M. Mastronardi, (Rubicon Genomics, Ann Arbor), M. Luo, M. Jeong, D. Sun, W. Lei, L. White, G. Darlington (Baylor College of Medicine, Houston) There is an increasing need to sequence picogram quantities of short DNA for diagnostics and research. For example, 1) maternal… Read more »

Methods to simplify template preparations and increase sensitivity of next-generation sequencing of short DNA and cDNA for non-invasive prenatal and cancer diagnostics as well as for FFPE-seq, ChIP-seq, and methyl-seq

J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor), M. Luo, M. Jeong, D. Sun, W. Lei, L. White, G. Darlington (Baylor College of Medicine, Houston) Small amounts of degraded and/or rare DNA are useful for prenatal and cancer diagnostic sequencing. Unfortunately, conventional NGS preps require ng or mg amounts of starting… Read more »

Two Methods for High-Throughput NGS Template Preparation for Small Clinical Samples Without Automation

J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor) Clinical samples are difficult to prepare for NGS, because of the small amounts of formalin-fixed tissue, plasma, urine, and single-cell DNA available from patients. Conventional whole genome amplification methods are too biased for NGS applications, and the existing NGS preparation kits require intermediate… Read more »

Experience with 2 methods to simplify NGS Preps of clinical samples, including FFPE tissue, plasma, immunoprecipitates and single cells

J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor) Formalin-fixed tissue, FNA, plasma, urine, CSF and other clinical samples were standardized for many scientific and practical reasons long before the value and limitations of genetic testing were known. These clinical samples are highly degraded, and the analytes present in limiting quantities (e.g.,… Read more »