Matthew Carroll1, Dmitry Goryunov1, Konstantinos Charizanis1, Fang Sun1, Maria Dinkelmann1, Edward Jan1, Kamran Shazand1, Emmanuel Kamberov1, Shawn Quinn2, John Langmore1 1Rubicon Genomics, Inc. • Ann Arbor, MI • 2 Curio Genomics, Inc. Dexter, MI Liquid biopsies provide a non-invasive method to acquire the genetic information provided in cell-free DNA. Next Generation Sequencing enables access to… Read more »
Resources by Category: "Plasma"
K. Shazand, J. Ning, E. Ranghini, A. Popkie, and J.P. Jerome The ability to detect rare alleles is key to the discovery and characterization of mutations in cancer research. These mutations are found at low frequencies, mainly because of tumor heterogeneity and tumor DNA dilution in the germline background. Cell-free DNA (cfDNA) in plasma provides… Read more »
Xia Y, Huang CC, Dittmar, R, Du M, Wang Y, Liu, H, Shenoy N, Wang L and Kohli M. Oncotarget, 2016 Apr 26. doi: 10.18632/oncotarget.9027. [Epub ahead of print]
Snyder MW, Kircher M, Hill AJ, Daza RM, Shendure J. Cell. 2016 Jan 14;164(1-2):57-68. DOI: 10.1016/j.cell.2015.11.050.
Julian, Colleen G, Ivana V Yang, Vaughn a Browne, Enrique Vargas, Carmelo Rodriguez, Brent S Pedersen, Lorna G Moore, and David a Schwartz. 2013. “Inhibition of Peroxisome Proliferator-Activated Receptor Γ: a Potential Link Between Chronic Maternal Hypoxia and Impaired Fetal Growth.” FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology 28 (Published online… Read more »
John Langmore, CSO, Rubicon Genomics Langmore Select Biosciences Genome Research 2012 (pdf)
J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor), M. Luo, M. Jeong, D. Sun, W. Lei, L. White, G. Darlington (Baylor College of Medicine, Advances in Genome Biology and Technology (pdf)
M. Mastronardi, T. Tesmer, E. Kamberov, J. Langmore Biofluid Challenges: Biofluids, including plasma, serum, urine and CSF, contain valuable biomarkers that are used in research and diagnostics. These matrices present many challenges to traditional sample preparation methods. Biofluid DNA is fragmented (100-200 bp) and very dilute (e.g., 1-20 ng/mL of plasma). Additionally, the biomarker-containing DNA… Read more »
American Society of Human Genetics 2011 (Poster – PDF) E. Kamberov, J. Langmore, T. Tesmer1, M. Mastronardi, (Rubicon Genomics, Ann Arbor), M. Luo, M. Jeong, D. Sun, W. Lei, L. White, G. Darlington (Baylor College of Medicine, Houston) There is an increasing need to sequence picogram quantities of short DNA for diagnostics and research. For example, 1) maternal… Read more »
J. Langmore, E. Kamberov, T. Tesmer, M. Mastronardi (Rubicon Genomics, Ann Arbor), M. Luo, M. Jeong, D. Sun, W. Lei, L. White, G. Darlington (Baylor College of Medicine, Houston) Small amounts of degraded and/or rare DNA are useful for prenatal and cancer diagnostic sequencing. Unfortunately, conventional NGS preps require ng or mg amounts of starting… Read more »